Poly(ethylene glycol)(PEG)-Protein Hybrids.
نویسندگان
چکیده
منابع مشابه
Intracellular protein interaction mapping with FRET hybrids.
A quantitative methodology was developed to identify protein interactions in a broad range of cell types by using FRET between fluorescent proteins. Genetic fusions of a target receptor to a FRET acceptor and a large library of candidate peptide ligands to a FRET donor enabled high-throughput optical screening for optimal interaction partners in the cytoplasm of Escherichia coli. Flow cytometri...
متن کاملChemical and Biological Sensing Utilizing Fused Bacteriorhodopsin Protein Hybrids
This paper describes how monomeric bR can be overproduced in Escherichia coli and subsequently utilized as an integral component of a generic, nanoscale chemical sensing platform. The utility of this sensing platform is that it can be adapted for detection of a wide range of biological and chemical agents at, or below, nanomolar concentration levels. The gene encoding for bacteriorhodopsin has ...
متن کاملProtein adsorption on various plasma-treated polyethylene terephthalate substrates.
Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate (PET) was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF(4) plasma to make the surface hydrophobic. The plasma source was radiofrequency (RF) discharge. The adsorption of albumin and other proteins from a cell-culture medium o...
متن کاملRecombinant Ganoderma lucidum immunomodulatory protein modified with polyethylene glycol.
Recombinant Ganoderma lucidum immunomodu-latory protein (rLZ-8) expressed using the Pichia yeast eukaryotic expression system is a potential new drug for cancer therapy; however, it has a short half-life in the body. In order to optimize the potency and stability of rLZ-8, we modified the recombinant protein chemically using methoxy-PEG-succinimid...
متن کاملMetaheuristic Hybrids
Over the last years, so-called hybrid optimization approaches have become increasingly popular for addressing hard optimization problems. In fact, when looking at leading applications of metaheuristics for complex real-world scenarios, many if not most of them do not purely adhere to one specific classical metaheuristic model but rather combine different algorithmic techniques. Concepts from di...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: KOBUNSHI RONBUNSHU
سال: 1991
ISSN: 0386-2186,1881-5685
DOI: 10.1295/koron.48.261